Chymosin (Rennet Enzyme) · Milk Coagulation Enzyme for Cheese Making and Dairy Processing

Chymosin is a milk-coagulating enzyme widely used in cheese production to initiate curd formation and support controlled gel development. It works by cleaving specific casein fractions, enabling the milk protein network to set in the presence of calcium and appropriate processing conditions. Industrial producers select chymosin grades based on activity, consistency, process fit and the desired balance between coagulation performance and proteolysis. Atlas Global Trading Co. supplies chymosin in liquid and powder options with agreed activity and specification targets, supported by technical documentation and export logistics from Ankara, Türkiye.

Function: Milk coagulation enzyme for curd formation in cheese production
Typical formats: Liquid or powder; activity units and dilution ratios as agreed
Key feature: Consistent coagulation behavior for standardized cheese making

Enzyme status, permitted use and labeling requirements vary by market and product category. Atlas provides documentation and supports grade selection, while customers remain responsible for confirming compliance and labeling in each destination market.

Product overview

Chymosin grades for cheese: activity, performance and process fit

Commercial chymosin is supplied with defined activity, handling characteristics and documentation suited to industrial dairy operations. Grade selection typically considers coagulation speed, curd firmness development, yield targets, and the desired ripening profile. Depending on supply chain and customer requirements, chymosin may be offered as fermentation-derived chymosin or other suitable production types, with specifications agreed case-by-case.

Key characteristics

  • Primary role: initiates milk coagulation for curd formation in cheese making.
  • Formats:
    • Liquid chymosin for dosing via pumps or calibrated additions,
    • Powder chymosin for dry storage and controlled reconstitution where applicable.
  • Activity and performance:
    • Supplied with activity expressed in industry units (e.g., IMCU or other agreed system),
    • Performance is influenced by milk composition, pH, calcium level, temperature and processing time.
  • Process focus:
    • Consistent coagulation time and curd firmness development,
    • Controlled proteolysis aligned with cheese style and ripening goals.

Atlas can help align chymosin activity and format to your plant conditions, cheese style and dosing equipment.

Indicative specification points

Specifications are agreed per grade and destination requirements. Typical control points include:

  • Enzyme activity: defined in the agreed unit system and measured by stated method.
  • Physical form: liquid (with defined density/viscosity) or powder (with defined solubility/flow).
  • pH (liquid grades): controlled for stability and dosing behavior.
  • Microbiological quality: targets appropriate for dairy processing under GMP.
  • Contaminants: parameters within required limits (food enzyme standards and customer requirements).
  • Stability and shelf life: stated on COA/specification and linked to storage conditions.

Each lot is supplied with a Certificate of Analysis (COA) and a specification sheet. Additional documents can include allergen statements, GMO status (where applicable), origin/production statements and other compliance documentation requested for destination markets.

Applications

Applications of chymosin in cheese manufacturing and dairy processing

Chymosin is used across many cheese types and dairy processes where controlled milk gelation is required. The correct grade and dosage depend on milk quality, plant conditions, cheese style and desired yield and texture targets. Pilot and line trials are recommended to optimize coagulation time, cutting window and curd handling.

Cheese types

  • Hard and semi-hard cheeses: supports consistent coagulation and curd firmness development.
  • Soft cheeses: enables controlled gel formation and cutting behavior under milder conditions.
  • Fresh cheeses: supports fast, repeatable curd formation for high-throughput production.
  • Regional and specialty cheeses: grade selection aligned with traditional process targets and ripening profiles.

Dairy process considerations

  • Milk standardization: fat/protein ratio and calcium balance influence coagulation performance.
  • Heat treatment: thermal history can change coagulation behavior; adjust conditions accordingly.
  • pH and temperature: key levers for coagulation speed and gel strength.

Usage and dosing guidance

  • Dosage: depends on activity, milk composition and target coagulation time; optimize through trials.
  • Addition method:
    • Liquid grades: typically diluted in clean, cool water for uniform distribution before addition,
    • Powder grades: reconstitute per supplier guidance and ensure full dissolution before use.
  • Mixing:
    • Ensure uniform dispersion in the vat to avoid local over- or under-dosing,
    • Standardize mixing time and agitation intensity to reduce batch variability.
  • Process control:
    • Track coagulation time and cutting point consistently (instrument or standardized manual method),
    • Validate yield, moisture and texture outcomes in finished cheese.
  • Regulatory and labeling:
    • Food enzyme labeling and processing-aid status vary by market; confirm the applicable framework.

Need a chymosin grade for your cheese line?

Share your cheese type, milk treatment, target coagulation time, vat size, dosing system, destination markets, annual volume and documentation needs. Atlas will propose suitable chymosin options and support your trials with practical guidance and QA/export documentation.

Discuss chymosin supply
Quality & supply

Quality documentation and global supply support for chymosin

Atlas Global Trading Co. coordinates chymosin supply through qualified partners and manages documentation and export logistics from Ankara, Türkiye, supporting industrial dairy plants, cheese producers and ingredient blenders worldwide.

Quality and documentation

  • Supplied as chymosin with agreed specification targets for activity, stability, microbiology and contaminants.
  • Each lot includes a Certificate of Analysis (COA) and specification sheet.
  • Documentation can include allergen statements, GMO status (where applicable), origin/production statements and compliance documents according to destination needs.
  • Food safety and quality certificates can be shared on request depending on supplier and market requirements.
  • Customers remain responsible for confirming permitted use and labeling in destination markets.

Packaging, storage and logistics

  • Standard packaging: liquid grades in sealed HDPE containers; powder grades in sealed bags or cartons (pack formats depend on grade and supplier).
  • Storage: keep tightly closed and follow temperature guidance stated on the label/specification to protect activity.
  • Handling: avoid contamination and moisture ingress; use good stock rotation.
  • Shelf life: stated on COA/specification; validate in your storage and distribution conditions.
  • Shipments: arranged under FOB, CFR, CIF or other agreed Incoterms with export documentation support coordinated from Ankara.

For a quotation, please share preferred format (liquid/powder), target activity unit system, packaging preference, annual volume and destination markets.

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